期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 50, 期 7, 页码 2234-2238出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.06504-11
关键词
-
类别
资金
- Federal Ministry of Education and Research
- Potential Release-Oriented Biothreat Emergency Diagnostics under the research program for civil security of the German Federal Government as part of a high-tech strategy for Germany
Several real-time PCR approaches to develop field detection for Francisella tularensis, the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard and DNA extracts from infected rabbit tissues. The assay showed a performance comparable to real-time PCR but reduced the assay time to 10 min. The rapid RPA method has great application potential for field use or point-of-care diagnostics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据