期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 49, 期 6, 页码 2314-2317出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00337-11
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资金
- Google.org
- National Institutes of Health (Northeast Biodefense Center) [AI57158]
- USAID (Center for Infection and Immunity) [07-301-7119-52258]
- NIH [NO1-AI-30027]
Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.
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