期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 48, 期 6, 页码 2075-2081出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.02509-09
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资金
- Fogarty International Research Cooperative [R03TW2711]
- International Research in Infectious Disease [AI075452]
- Global Infectious Disease Research Training [D43 TW007392]
Cryptosporidium spp., a common cause of diarrhea in children, were investigated in the first multisite study in India. Diarrheal stools from hospitalized children aged <5 years from Delhi, Trichy, and Vellore were analyzed by microscopy, PCR-restriction fragment length polymorphism (RFLP), and/or sequencing at the small-subunit (SSU) rRNA and Cpgp40/15 loci for species determination and subgenotyping, respectively. Seventy of 2,579 (2.7%) children, 75% of whom were <2 years old, had cryptosporidial diarrhea as determined by microscopy. Genotyping and subgenotyping showed that Cryptosporidium hominis was the most commonly identified species (59/67 children), and subgenotypes Ie, Ia, Ib, and Id were common in all centers. A novel C. parvum subgenotype, IIn, was identified in Vellore. Meteorological analysis revealed a higher rate of cryptosporidial positivity during hotter and drier weather in Delhi.
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