期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 48, 期 10, 页码 3482-3486出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00687-09
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类别
资金
- NIH Clinical Center
- Department of Laboratory Medicine
We evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the rapid identification of yeast species. Using Bruker Daltonics MALDI BioTyper software, we created a spectral database library with m/z ratios of 2,000 to 20,000 Da for 109 type and reference strains of yeast (44 species in 8 genera). The database was tested for accuracy by use of 194 clinical isolates (23 species in 6 genera). A total of 192 (99.0%) of the clinical isolates were identified accurately by MALDI-TOF MS. The MALDI-TOF MS-based method was found to be reproducible and accurate, with low consumable costs and minimal preparation time.
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