期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 47, 期 6, 页码 1906-1910出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00629-09
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- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Ministry of Health, Labor, and Welfare of Japan
We developed an efficient cell culture system for genotype 4 hepatitis E virus using the HE-JF5/15F strain recovered from a fulminant hepatitis patient. The sixth-passage virus in the culture supernatant reached 1.5 x 108 copies/ml at 10 days postinoculation and possessed 10 nucleotide mutations with four amino acid changes.
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