期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 47, 期 5, 页码 1562-1564出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00124-09
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资金
- U.S.Army Medical Research and Materiel Command [PR054228]
- Office of Congressionally Directed Medical Research Programs
- National Institutes of Health [N01-AI-30041]
- NIDCR [DE14318]
Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.
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