期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 47, 期 3, 页码 674-679出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01028-08
关键词
-
类别
资金
- Federal Ministry of Food, Agriculture and Consumer Protection [FSI 1-1.1.2.]
The spread of highly pathogenic avian influenza A virus (HPAIV) of subtype H5N1 demands fast and reliable methods for in-depth, full-length sequence analysis. For this purpose, we designed a simple and sensitive method for the preparation of sequencing libraries from H5N1 HPAIV diagnostic RNA samples for sequencing with the Genome Sequencer FLX instrument. The method presented seamlessly integrates high-throughput pyrosequencing with the Roche/454 instrument into diagnostics without the need for additional equipment or molecular biological techniques besides standard PCR and the Genome Sequencer FLX sample preparation and sequencing pipeline.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据