期刊
JOURNAL OF CLINICAL INVESTIGATION
卷 124, 期 5, 页码 2136-2146出版社
AMER SOC CLINICAL INVESTIGATION INC
DOI: 10.1172/JCI70577
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资金
- Fondation Leducq (MIRVAD)
- IFB-Tx [01EO1302]
- DFG [TH903/10-1]
- REBIRTH Excellence Cluster
- Hannover Biomedical Research School program Molecular Medicine
- EU [FP7-CIG-294278]
- FIBROTARGET
- German Federal Ministry of Education and Research [0315690D]
- British Heart Foundation [SP/12/5/29574, FS/13/2/29892] Funding Source: researchfish
In response to stress, the heart undergoes extensive cardiac remodeling that results in cardiac fibrosis and pathological growth of cardiomyocytes (hypertrophy), which contribute to heart failure. Alterations in microRNA (miRNA) levels are associated with dysfunctional gene expression profiles associated with many cardiovascular disease conditions; however, miRNAs have emerged recently as paracrine signaling mediators. Thus, we investigated a potential paracrine miRNA crosstalk between cardiac fibroblasts and cardiomyocytes and found that cardiac fibroblasts secrete miRNA-enriched exosomes. Surprisingly, evaluation of the miRNA content of cardiac fibroblast-derived exosomes revealed a relatively high abundance of many miRNA passenger strands (star miRNAs), which normally undergo intracellular degradation. Using confocal imaging and coculture assays, we identified fibroblast exosomal-derived miR-21_3p (miR-21*) as a potent paracrineacting RNA molecule that induces cardiomyocyte hypertrophy. Proteome profiling identified sorbin and SH3 domain-containing protein 2 (SORBS2) and PDZ and LIM domain 5 (PDLIM5) as miR-21* targets, and silencing SORBS2 or PDLIM5 in cardiomyocytes induced hypertrophy. Pharmacological inhibition of miR-21* in a mouse model of Ang II-induced cardiac hypertrophy attenuated pathology. These findings demonstrate that cardiac fibroblasts secrete star miRNA-enriched exosomes and identify fibroblast-derived miR-21* as a paracrine signaling mediator of cardiomyocyte hypertrophy that has potential as a therapeutic target.
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