期刊
JOURNAL OF CLINICAL INVESTIGATION
卷 120, 期 5, 页码 1415-1428出版社
AMER SOC CLINICAL INVESTIGATION INC
DOI: 10.1172/JCI40494
关键词
-
资金
- Region Rhone-Alpes
- CNRS
- INSERM
- Association pour la Recherche sur le cancer [3977]
- Agence Nationale de la Recherche
- Ministry of Education, Science, Culture, Sports and Technology of Japan
- Japan Science and Technology Agency
- NOVARTIS Foundation for the Promotion of Science
- Toray Science Foundation
- Grants-in-Aid for Scientific Research [21390036, 21390027] Funding Source: KAKEN
Ejaculated mammalian sperm must undergo a maturation process called capacitation before they are able to fertilize an egg. Several studies have suggested a role for members of the secreted phospholipase Ay (sPLA(2)) family in capacitation, acrosome reaction (AR), and fertilization, but the molecular nature of these enzymes and their specific roles have remained elusive. Here, we have demonstrated that mouse group X sPLA(2) (mGX) is the major enzyme present in the acrosome of spermatozoa and that it is released in an active form during capacitation through spontaneous AR. mGX-deficient male mice produced smaller litters than wild-type male siblings when crossed with mGX-deficient females. Further analysis revealed that spermatozoa from mGX-deficient mice exhibited lower rates of spontaneous AR and that this was associated with decreased in vitro fertilization (IVF) efficiency due to a drop in the fertilization potential of the sperm and an increased rate of aborted embryos. Treatment of sperm with sPLA(2) inhibitors and antibodies specific for mGX blocked spontaneous AR of wild-type sperm and reduced IVF success. Addition of lysophosphatidylcholine, a catalytic product of mGX, overcame these deficiencies. Finally, recombinant mGX triggered AR and improved IVF outcome. Taken together, our results highlight a paracrine role for mGX during capacitation in which the enzyme primes sperm for efficient fertilization and boosts premature AR of a likely phospholipid-damaged sperm subpopulation to eliminate suboptimal sperm from the pool available for fertilization.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据