期刊
JOURNAL OF CLINICAL INVESTIGATION
卷 119, 期 3, 页码 565-572出版社
AMER SOC CLINICAL INVESTIGATION INC
DOI: 10.1172/JCI37865
关键词
-
资金
- Wellcome Trust
- Biotechnology and Biological Sciences Research Council (BBSRC).
Th17 cells are involved in. the pathogenesis of many autoimmune diseases, but it is not clear whether they play a pathogenic role in type I diabetes. Here we investigated whether mouse Th17 cells with specificity for an islet antigen can induce diabetes upon transfer into NOD/SCID recipient mice. Induction of diabetes in NOD/SCID mice via adoptive transfer of Th1 cells from BDC2.5 transgenic mice was prevented by treatment of the recipient mice with a neutralizing IFN-gamma-specific antibody. This result suggested a major role of Th1 cells in the induction of disease in this model of type I diabetes. Nevertheless, transfer of highly purified Th17 cells from BDC2.5 transgenic mice caused diabetes in NOD/SCID recipients with similar rates of onset as in transfer of Th1 cells. However, treatment with neutralizing IL-17-specific antibodies did not prevent disease. Instead, the transferred Th17 cells, completely devoid of IFN-gamma at the time of transfer, rapidly converted to secrete IFN-gamma in the NOD/SCID recipients. Purified Th17 cells also upregulated Tbet and secreted IFN-gamma upon exposure to IL-12 in vitro and in vivo in NOD/SCID recipients. These results indicate substantial plasticity of Th17 commitment toward a Th1-like profile.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据