Overfeeding Over 24 Hours Does Not Activate Brown Adipose Tissue in Humans

Context: Human brown adipose tissue (BAT) is activated with cold exposure, but it is unknown whether overfeeding activates BAT. Objective: We determined BAT activation with cold, fasting, and overfeeding and the relationship of BAT activation with future weight change. Design, Setting, Participants, and Interventions: Sixteen healthy adults were evaluated during energy balance, fasting, and 24 hours of 200% overfeeding. All subjects had a fluorodeoxyglucose-positron emission tomography (PET) scan after exposure to 16 degrees C to determine cold-induced BAT activity (CIBA). The first six subjects had a second PET scan after 36 hours of fasting to establish the lack of BAT activation at 22 degrees C. The other subjects' second PET scan occurred after 24 hours of overfeeding at 22 degrees C but only if they demonstrated CIBA. Twelve subjects returned at 6 months for reassessment of body composition. Main Outcome Measures: BAT was defined in cool scans as voxels with a standardized uptake value (SUV) of 2.0 or greater and Hounsfield units between -250 and -10. Body composition was assessed by dual-energy x-ray absorptiometry. Results: Although 75% of the subjects demonstrated visible CIBA, none had visual BAT activity after overfeeding. CIBA was greater than that observed in the same defined BAT voxels after fasting (n = 6; 2.9 +/- 0.5 vs 1.2 +/- 0.2; Delta = -1.7; 95% confidence interval -2.4, -1.0 SUV; P < .01). In the second cohort, CIBA was also higher than observed BAT voxel activity after 24 hours overfeeding (n = 8; 3.5 +/- 0.7 vs 0.9 +/- 0.2; Delta = -2.6; 95% confidence interval -3.2, -1.9 SUV; P < .01). Baseline CIBA negatively correlated with changes in fat mass after 6 months (r = -0.72, P = .009). Conclusions: BAT may be important in weight regulation unrelated to the response to overeating.