Improvement of Vitamin D Status via Daily Intake of Fortified Yogurt Drink Either with or without Extra Calcium Ameliorates Systemic Inflammatory Biomarkers, including Adipokines, in the Subjects with Type 2 Diabetes

Context: Systemic inflammation is thought to have a central role in diabetic long-term complications. Objective: The aim of this study was to investigate the effects of vitamin D either with or without extra calcium on certain inflammatory biomarkers in the subjects with type 2 diabetes (T2D). Design, Setting, and Participants: This was a double-blind, randomized, controlled trial conducted over 12 wk in 90 T2D subjects aged 30-60 yr from both sexes. Intervention: Subjects were randomly allocated to one of three groups to receive two 250-ml bottles a day of plain Persian yogurt drink or doogh (PD, containing 150 mg calcium and no detectable vitaminD(3)/250 ml), vitamin D-fortifieddoogh(DD, containing 500 IU vitamin D-3 and 150 mg calcium/250 ml), or calcium + vitamin D-3-fortified doogh (CDD, containing 500 IU vitamin D3 and 250 mg calcium/250 ml). Outcome Measures: The changes in inflammatory markers were evaluated. Results: Comparedto the baseline values, highly sensitive C-reactive protein, IL-1 beta, IL-6, fibrinogen, and retinol binding protein-4 concentrations significantly decreased in both the DD and CDD groups. Although the decrement in highly sensitive C-reactive protein and fibrinogen was more in CDD compared to DD (-4.0 +/- 8.5 vs. -1.3 +/- 2.8 mg/liter, and -0.40 +/- 0.74 and -0.20 +/- 0.52 mg/liter, respectively), the differences were not significant. There was a significant increase in serum adiponectin in both the DD and CDD groups (51.3 +/- 65.3 vs. 57.1 +/- 33.8 mu g/liter; P < 0.05). Mean adiponectin changes in CDD were significantly higher than in PD (P = 0.021). Conclusions: Daily intake of vitamin D-fortified doogh improved inflammatory markers in T2D subjects, and extra calcium conferred additional benefit only for the antiinflammatory adipokine, i.e. adiponectin. (J Clin Endocrinol Metab 97: 2005-2011, 2012)