期刊
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
卷 93, 期 5, 页码 1980-1987出版社
ENDOCRINE SOC
DOI: 10.1210/jc.2007-2235
关键词
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资金
- NCRR NIH HHS [K23 RR018770, R01 RR019991, M01 RR000847, M01 RR 00847, K23 RR 018770, R01 RR019991-05] Funding Source: Medline
- NIDDK NIH HHS [R01 DK 32632, R01 DK076037, R25 DK064122-05, R25 DK064122] Funding Source: Medline
Context: Ghrelin, an acylated peptide hormone secreted from the gut, regulates appetite and metabolism. Elucidating its pattern of secretion in the fed and fasted states is important in the face of the obesity epidemic. Objective: Our objective was to examine changes in circulating ghrelin and des-acyl ghrelin in response to meals and fasting using newly developed two-site sandwich assays and sample preservation protocols to allow specific detection of full-length forms. Design: Ten-minute sampling was done for 26.5 h during a fed admission with standardized meals and on a separate admission during the final 24h of a 61.5-h fast and continuing for 2.5h after terminating the fast. Setting: The study was conducted at the University Hospital General Clinical Research Center. Participants: Eight male volunteers participated, mean +/- SD age 24.5 +/- 3.7 yr and body mass index 24 +/- 2.1 kg/m(2). Main Outcome Measures: Ten-minute sampling profiles were assessed for ghrelin and des-acyl ghrelin, fed and fasting. Results: In the fed state, ghrelin and des-acyl ghrelin showed similar dynamics; both were sharply inhibited by meals and increased at night. During fasting, ghrelin decreased to nadir levels seen postprandially, and des-acyl ghrelin remained near peak levels seen preprandially. Total full-length ghrelin (acyl plus des-acyl) levels remained unchanged. Conclusions: Meals inhibited secretion of both ghrelin and des-acyl ghrelin, yet long-term fasting inhibited acylation but not total secretion. Acylation may be regulated independently of secretion by nutrient availability in the gut or by esterases that cleave the acyl group. These studies highlight the importance of stringent conditions for sample collection and evaluation of full-length ghrelin and des-acyl ghrelin using specific two-site assays.
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