期刊
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
卷 93, 期 6, 页码 2375-2382出版社
ENDOCRINE SOC
DOI: 10.1210/jc.2008-0136
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资金
- Medical Research Council [G0500717] Funding Source: researchfish
- Medical Research Council [G0500717] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
Context: Ectopic pregnancy is common but remains difficult to diagnose accurately. There is no serum test to differentiate ectopic from intrauterine gestation. Objective: Our objective was to investigate differential gene expression in decidualized endometrium of ectopic pregnancy. Design: Tissue and serum analysis informed by microarray study was performed. Setting: The study was performed at a large United Kingdom teaching hospital. Patients or Other Participants: Women undergoing surgical termination of pregnancy (n = 8), evacuation of uterus for miscarriage (n = 6), and surgery for tubal ectopic pregnancy (n = 11) were included in the study. Endometrium was collected from normally cycling women undergoing hysterectomy. Interventions: Decidualized endometrium was subjected to microarray analysis, morphological assessment, and immunohistochemistry. Endometrial stromal fibroblasts were cultured in the presence of decidualizing stimuli. Main Outcome Measures: Differential expression of potentially secreted molecules was calculated. Results: Inhibin/activin beta-B expression was lower in decidualized endometrium from ectopic pregnancies when compared with that of ongoing pregnancies (P < 0.01) or miscarriages (P < 0.01). The localization of the beta-B subunit was more marked in decidualized than nondecidualized stroma. Decidualization of stromal fibroblasts in vitro was associated with increased beta-B expression (P < 0.05). Endometrial stroma of ectopic pregnancies was less decidualized morphologically (P < 0.05), with lower prolactin (P < 0.01) and IGF binding protein-1 (P < 0.005) expression. Serum activin B was lower in ectopic pregnancies (P < 0.005) than in intrauterine pregnancies, whereas there was no difference in progesterone concentrations. Conclusions: Despite similar concentrations of progesterone, the endometrium of ectopic pregnancies is less decidualized than intrauterine pregnancies. Expression of the beta-B subunit is related to decidualization and can be detected in the circulation as activin B. Serum activin B concentrations are lower in ectopic pregnancy.
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