期刊
NEUROSCIENCE
卷 289, 期 -, 页码 214-223出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2014.12.054
关键词
diabetes; white matter lesion; oligodendrocyte progenitors; chronic ischemia; dementia
资金
- Research Institute for Disease of Old Age, Juntendo University, Tokyo
- JSPS KAKENHI [25430053]
- Grants-in-Aid for Scientific Research [25430053] Funding Source: KAKEN
Diabetes mellitus (DM) is a major risk factor for stroke and it exacerbates tissue damage after ischemic insult. Diabetes is one of the important causes of the progression of white matter lesion, however, the pathological mechanisms remain unclear. The present study evaluated the influences of type 2 DM on ischemic subcortical white matter injury and the recruitment of oligodendrocyte progenitor cells (OPCs) under chronic cerebral hypoperfusion using type 2 diabetic (db/db) mice. After bilateral common carotid artery stenosis (BCAS), the rarefaction in the white matter was more severe in db/db mice than in db/+ mice, and the number of glutathione S-transferase-pi (GST-pi)positive mature oligodendrocytes (OLG) was lower in db/db mice than in db/+ mice at 4 and 8 weeks after ischemia. There were no significant differences in the number of single-stranded DNA (ssDNA)-positive apoptotic cells in the deep white matter between the db/db and db/+ mice. We found a transient increase in the platelet-derived growth factor receptor-alpha (PDGFR alpha)-positive OPCs in white matter lesions after ischemia. However, significantly fewer PDGFR alpha-positive OPCs were detected in db/db than db/+ mice from 4 weeks after BCAS. The number of Ki67-positive proliferating cells in the deep white matter was significantly lower in db/db mice than in db/+ mice from 4 to 8 weeks after BCAS. Most of the Ki67-positive cells were PDGFR alpha-positive OPCs. Finally, we assessed the survival of 5-bromo-20-deoxyuridine (BrdU)-positive proliferating cells in ischemic white matter, and found significantly poorer survival of BrdU/PDGFR alpha-positive OPCs or BrdU/GST-pi-positive OLGs in the db/db mice compared to the db/+ mice in the white matter after BCAS. Our findings suggest that the type 2 DM mice exhibited more severe white matter injury 8 weeks after chronic ischemia. Decreased proliferation and survival of OPCs may play an important role in the progression of white matter lesions after ischemia in diabetics. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.
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