期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 945, 期 -, 页码 163-170出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2013.11.053
关键词
Xanthine oxidase; Magnetic nanoparticles; Selective fishing; Puerariae lobata flower; Glycyrrhiza uralensis root; HPLC-MS
资金
- National Natural Science Foundation of China [21175155, 21275163]
- Science and Technology Program of Hunan Province [2012FJ2006]
- Hunan Provincial Innovation Foundation for Postgraduate [CX2012B119]
- Fundamental Research Funds for the Central Universities of Central South University [2012zzts003]
- Students Innovation Training of Central South University [LC12131]
Xanthine oxidase (XOD) immobilized core-shell magnetic silica (Fe3O4@SiO2-XOD) nanoparticles coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) was developed to fish out and analyze XOD binders from two Fabaceae species, Puerariae lobata flower and Glycyrrhiza uralensis root. The prepared Fe3O4@SiO2-XOD nanoparticles exhibited good specificity for XOD binders, better dispersion in aqueous solution and reusability than those of Fe3O4-XOD nanoparticles. The amount of XOD immobilized onto Fe3O4@SiO2 nanoparticles was 339.9 mu g/mg and the activity of Fe3O4@SiO2-XOD nanoparticles remained 95% after ten times usage. The optimum conditions of selective fishing were optimized, and finally incubating pH was set at 7, incubating temperature at 25 degrees C and adsorption time at 30 min. Twelve XOD binders were successfully identified from ethyl acetate extract of P. lobata flower and G. uralensis root. The developed method provides a rapid, purposeful and effective way to identify active compounds from natural complex mixtures. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据