期刊
出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2014.01.055
关键词
Cloud point extraction; HPLC; Aristolochic acids; Genapol X-080; Rat plasma; Aristolochiae Fructus
资金
- National Natural Science Foundation of China [81060326, 81260605]
- Jiangxi Provincial Natural Science Foundation [2010GZY0163]
- Jiangxi Provincial Education Department [GJJ09277]
Based on cloud-point extraction (CPE), a high performance liquid chromatography method (HPLC) was developed and validated for the determination of aristolochic acids (AAs) in rat plasma after oral administration of Aristolochiae Fructus (AF). Non-ionic surfactant Genapol X-080, an environmentally friendly solvent, was used for the micelle-mediated extraction. Various influencing factors on CPE process were investigated and optimized. AAs were extracted from rat plasma after adding 1 ml of 4.5% (v/v) surfactant in the presence of 0.2 mol/l HCl and 20 mg NaCl, and the incubation temperature and time were 50 degrees C and 10 min, respectively. Base-line separation was obtained for the AAs in rat plasma with the optimized chromatography conditions. The detection limits (LOD) reached downward 10 ng/ml. The intra-day and inter-day precisions were less than 7.8%, the accuracies were within +/-5.5%, and the average recovery factors were in the range of 94.5-105.4%. In comparison with liquid-liquid extraction, the CPE method has a considerable LOD and higher recoveries. The proposed CPE-HPLC method was specific, sensitive and reliable, and could be an effective tool for the determination of AAs in biological matrixes. With the method the pharmacokinetics of AAs were investigated successfully after oral administration of AF by rats. (C) 2014 Elsevier B.V. All rights reserved.
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