期刊
出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2014.01.026
关键词
Affinity microcolumns; High-performance affinity chromatography; Biointeraction analysis; Zonal elution; Frontal affinity chromatography; Ultrafast affinity extraction
资金
- NIH [R01 GM044931, R01 DK069629]
- NSF REU program
- NSF/EPSCoR program [EPS-1004094]
- Molecular Mechanisms of Disease program at the University of Nebraska
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1156560] Funding Source: National Science Foundation
- EPSCoR
- Office Of The Director [1004094] Funding Source: National Science Foundation
Affinity chromatography has become an important tool for characterizing biomolecular interactions. The use of affinity microcolumns, which contain immobilized binding agents and have volumes in the mid-to-low microliter range, has received particular attention in recent years. Potential advantages of affinity microcolumns include the many analysis and detection formats that can be used with these columns, as well as the need for only small amounts of supports and immobilized binding agents. This review examines how affinity microcolumns have been used to examine biomolecular interactions. Both capillary-based microcolumns and short microcolumns are considered. The use of affinity microcolumns with zonal elution and frontal analysis methods are discussed. The techniques of peak decay analysis, ultrafast affinity extraction, split-peak analysis, and band-broadening studies are also explored. The principles of these methods are examined and various applications are provided to illustrate the use of these methods with affinity microcolumns. It is shown how these techniques can be utilized to provide information on the binding strength and kinetics of an interaction, as well as on the number and types of binding sites. It is further demonstrated how information on competition or displacement effects can be obtained by these methods. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据