期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 965, 期 -, 页码 231-237出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2014.06.037
关键词
Simulated moving bed chromatography; Cyclolinopeptide; Orbitide; Separation; Flow rate
资金
- Saskatchewan Ministry of Agriculture
- Canada Foundation for Innovation
- Agriculture and AgriFood Canada
- Genome Canada of TUFGEN (Total Utilization Flax Genomics)
The purification and enrichment of most natural products with potential pharmaceutical applications has been performed mainly employing conventional batch-mode chromatographic processes. There is a growing interest in use of simulated moving bed (SMB) chromatography for natural product enrichment as this method enables conservation of mobile phase, while increasing productivity of chromatography medium. SMB increases yield while decreasing cost. Cyclolinopeptides C ([1-9-NaC],[1-MetpO]-CLB, 3) and E([1-8-NaC],[1-MetO]-CLE, 8) were extracted as a mixture from flaxseed oil and then enriched using a three-zone simulated moving bed. The current research extends the SMB technology to enrichment of cyclolinopeptides (CLs), a group of biologically active hydrophobic cyclic peptides that occur in flaxseed oil. Of interest are [1-9-NaC],[1-MetO]-CLB (3) and [1-8-NaC],[1-MetO]-CLE (8) that provide synthetic scaffolds for modified CLs. The influence of flow rate (feed, desorbent, and extract) on the separation of [1-9-NaC],[1-MetO]-CLB (3) and [1-8-NaC],[1-MetO]-CLE (8) was investigated. (C) 2014 Elsevier B.V. All rights reserved.
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