期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 927, 期 -, 页码 142-146出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2013.01.003
关键词
3-Deazaneplanocin; Ultra HPLC-MS/MS; Pharmacokinetics
资金
- Intramural NIH HHS [ZIC SC006536-19] Funding Source: Medline
3-Deazaneplanocin A (DZNep) has been shown to have anti-cancer activity in numerous cancer types and its continued preclinical, and eventual clinical, drug development will require rapid and sensitive bioanalytical methods in order to quantitate this drug for pharmacokinetic analyses. The ultra HPLC with positive thermospray tandem mass spectrometric (LC-MS/MS) detection affords the most sensitive (limit of quantitation 5 ng/mL) and rapid (3 min run time) bioanalytical method to date for DZNep. Due to the polar nature of this drug and the internal standard (tubercidin), a hydrophilic-interaction column (HILIC) was used. The method was accurate, with less than 10% deviation from nominal values, as well as precise, where both within-day and between-day precisions were less than 15%. A liquid-liquid extraction procedure was able to recover similar to 90% of drug from a small volume (50 mu L) of mouse plasma. This method was successfully applied to a pharmacokinetic study in mice intravenously injected with DZNep. Published by Elsevier B.V.
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