期刊
出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2012.10.026
关键词
Reversed-phase ion-pairing chromatography; Electrospray ionization-mass spectrometry; Dopamine oxidation; 6-Hydroxydopamine; Short-lived intermediates
资金
- Central South University from the National Institutes of Health [SC1NS070155-01]
- National Science Foundation (NSF) [1112105]
- NSF-CREST Program at California State University, Los Angeles [NSF HRD-0931421]
- Direct For Education and Human Resources
- Division Of Human Resource Development [0932421] Funding Source: National Science Foundation
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1112105, 1062427] Funding Source: National Science Foundation
Reversed-phase ion-pairing chromatography (RP-IPC) is coupled on-line with electrospray ionization-mass spectrometry (ESI-MS) through an interface comprising a four-way switch valve and an anion exchange column. Regeneration of the anion exchange column can be accomplished on-line by switching the four-way switch valve to interconnect the column to a regeneration solution. Positioning the anion exchange column between the RP-IPC and ESI-MS instruments allows the ion-pairing reagent (IPR) sodium octane sulfonate to be removed. The IPC-ESI-MS method enabled us to separate and detect four intermediates of the Fe(III)-catalyzed dopamine oxidation. In particular, 6-hydroxydopamine, which is short-lived and highly neurotoxic, was detected and quantified. Together with the separation of other intermediates, gaining insight into the mechanism and kinetics of the Fe(III)-catalyzed dopamine oxidation becomes possible. Published by Elsevier
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