期刊
出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2012.09.032
关键词
Protocatechuic acid; LC-MS/MS; Pharmacokinetics; Plasma
资金
- National Institute of Health (NIH) [R21 CA132138, RC2 CA148099]
- Biomedical Mass Spectrometric Laboratory at The Ohio State University
Protocatechuic acid (PCA), a major microbial-mediated metabolite of anthocyanins, has significant anti-oxidative and anti-carcinogenic activities in vitro and in vivo: however, its pharmacokinetics remains largely unknown. In this report, a sensitive and rapid LC-MS/MS method was developed and validated for the measurement of PCA concentrations in both mouse and human plasma. This method showed a linearity of 1-1000 ng/mL in both mouse and human plasma with a lower limit of quantification of 1 ng/mL. The within-day and between-day coefficient of variation ranged from 1.18 to 11.8% and accuracy from 92 to 110%. The method was applied to characterize the pharmacokinetics of PCA in mice after oral administration of 50 mg/kg PCA. PCA was absorbed rapidly with a half-life of 2.9 min, reached a peak plasma level (C-max) of 73.6 mu M at 5 min, and remained detectable up to 8h with the initial elimination half-life of about 3 min and a terminal half-life of 16 min. The area under the plasma concentration-time curve (AUC(0 -> 8 h)) of PCA was 1456 mu M min. The method was capable of detecting low ng/mL quantities of PCA in the plasma of patients with prostate cancer after an oral ingestion of 60 g of black raspberry (BRB) powder. Because PCA is derived from the anthocyanins in BRB, our method provides a useful analytical tool to further investigate the metabolism of anthocyanins, and the pharmacology of PCA in future pre-clinical and clinical studies. Published by Elsevier B.V.
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