期刊
出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2012.10.023
关键词
Saliva; Alpha-amylase; Liquid chromatography; Mass spectrometry; Proteolysis; Biomarker
资金
- NHMRC [631615]
- Career Development Fellowship [APP1031542]
- Queensland Government Smart Futures Fellowship
- University of Queensland Foundation Research Excellence Award
Saliva is a crucial biofluid for oral health and is also of increasing importance as a non-invasive source of disease biomarkers. Salivary alpha-amylase is an abundant protein in saliva, and changes in amylase expression have been previously associated with a variety of diseases and conditions. Salivary alpha-amylase is subject to a high diversity of post-translational modifications, including physiological proteolysis in the oral cavity. Here we developed methodology for rapid sample preparation and non-targeted LC-ESI-MS/MS analysis of saliva from healthy subjects and observed an extreme diversity of alpha-amylase proteolytic isoforms. Our results emphasize the importance of consideration of post-translational events such as proteolysis in proteomic studies, biomarker discovery and validation, particularly in saliva. (C) 2012 Elsevier B.V. All rights reserved.
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