期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 879, 期 5-6, 页码 317-325出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2010.12.016
关键词
Microdialysis; UPLC-Q-TOF/MS; Blood; Kidney; Abelmoschus manihot extract
资金
- Construction Project for Jiangsu Key Laboratory for High Technology Research of TCM Formulae [BM2010576]
- Construction Project for Jiangsu Engineering Center of Innovative Drug from Blood-conditioning TCM Formulae
- Practical and Innovative Training Program of College Students in Jiangsu Province
In this paper, microdialysis combining with ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was applied to simultaneously identify components in blood and kidney dialysis after oral administration of Abelmoschus manihot extract. Microdialysis probe was implanted in the jugular vein and the kidney medulla, respectively; microdialysis samples were collected continuously, transferred to microtubes and analyzed by UPLC-Q-TOF/MS. The components in microdialysis samples were separated by an UPLC HSS T3 column and eluted with acetonitrile and water (containing 0.1% formic acid) at a flow rate of 0.4 mL/min. The results showed that unbound constituents in blood circulation of the rat include hyperoside, isoquercitrin, quercetin monoglucuronide, quercetin-3'-O-glucoside, quercetin, myricetin, and hibifolin while unbound constituents in kidney are hyperoside, isoquercitrin, quercetin monoglucuronide, which might be the potential active components in vivo. The developed method was simple and reliable, and could be adopted to rapidly screen and identify potential active components contributing to pharmacological effects of TCM and to better clarify its action mechanism. (C) 2010 Elsevier B.V. All rights reserved.
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