期刊
出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2010.11.017
关键词
Solid-phase extraction; Dispersive liquid-liquid microextraction; Clenbuterol; Tissue samples; Liquid chromatoraphy
资金
- National Natural Science Foundation of China [20905019, 21011140338]
- Natural Science Foundation of Hebei [B2010000209]
A new pretreatment method, solid-phase extraction combined with dispersive liquid-liquid microextration (SPE-DLLME), was proposed in first time for the determination of clenbuterol (CLB) in porcine tissue samples. The tissue samples were firstly extracted by SPE, then its eluents were used as dispersant of the followed DLLME for further purification and enrichment of CLB. Various parameters (such as the type of SPE sorbent, the type and volume of elution solvent, the type and volume of extractant and dispersant, etc.) that affected the efficiency of the two steps were optimized. Good linearity of CLB was ranged from 0.19 mu g/kg to 192 mu g/kg with correlation coefficient (r(2)) of 0.9995. The limit of detection (LOD) was 0.07 mu g/kg (S/N = 3) and the recoveries at three spiked levels were ranged from 87.9% to 103.6% with the relative standard deviation (RSD) less than 3.9% (n = 3). Under the optimized conditions, the enrichment factor (EF) for CLB could up to 62 folds. The presented method that combined the advantages of SPE and DLLME, had higher selectivity than SPE method and was successfully applied to the determination of CLB in tissue samples. (C) 2010 Elsevier B.V. All rights reserved.
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