A new acid mix enhances phosphopeptide enrichment on titanium- and zirconium dioxide for mapping of phosphorylation sites on protein complexes

The selective enrichment of phosphorylated peptides prior to reversed-phase separation and mass spectrometric detection significantly improves the analytical results in terms of higher number of detected phosphorylation sites and spectra of higher quality. Metal oxide chromatography (MOC) has been recently described for selective phosphopeptide enrichment (Pinkse et al, 2004 [1]; Larsen et al, 2005 [2]: Kweon and Hakansson, 2006 [3]. Cantin et al, 2007 141, Collins et al., 2007 151). In the present work we have tested the effect of a modified loading solvent containing a novel acid mix and optimized wash conditions oil the efficiency of TiO2-based phosphopeptide enrichment in order to improve our previously published method (Mazanek et al. 2007 161). Applied to a test mixture of synthetic and BSA-derived peptides, the new method showed improved selectivity for phosphopeptides, whilst retaining a high recovery rate Application of the new enrichment method to digested purified protein complexes resulted in the identification of a significantly higher number of phosphopeptides as compared to the previous method Additionally. we have compared the performance of TiO2 and ZrO2 columns for the isolation and identification of phosphopeptides from purified protein complexes and Found that for out test set. both media performed comparably well In summary, Out improved method is highly effective for the enrichment of phosphopeptides from purified protein complexes prior to mass spectrometry, and is suitable for large-scale phosphoproteomic projects that aim to elucidate phosphorylation-dependent cellular processes. (C) 2009 Elsevier B.V. All rights reserved.