期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 878, 期 2, 页码 278-282出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2009.06.007
关键词
Immunoaffinity monolith; Class-specific; Pyrethroid; On-line clean-up
资金
- National Natural Science Foundation of China [90717002, 20675006, 30771425]
- National 863 Program [2007AA06Z401]
A class-specific monolithic immunoaffinity column was developed for on-line clean-up of pyrethroid insecticides in complex samples Deltamethrin was oxidized with ozone to generate the hapten of (RS)-acyano-alpha-phenoxybenzyl (RS)-cis,traps-2,2-dimethyl-3-formyl-cyclopropane carboxylate Class-specific antibodies against pyrethroids were produced using the conjugate of above hapten with bovine serum albumin as the immunogen. Poly(ethylene dimethacrylate-glycidyl methacrylate) monolith was synthesized in a 50 mm, 4.6 mm id stainless steel cartridge with two auxiliary pipette tips. The polymerization method was proved to be economic and reproducible. Antibodies against pyrethrotds were covalently immobilized onto the monolithic support via Schiff base reaction With a column-switching valve system, the immunoaffinity monolith (IAM) could be readily adapted to the reversed-phase high-performance liquid chromatography (HPLC) system. Under the optimum loading, washing and eluting conditions, the IAM specifically retained deltamethrin, flumethrin. flucythrinate and cis/traps permethrin, which were further baseline separated by C18 column using acetonitrile-water (83:17, V/V) as the mobile phase at a flow rate of 1.0 mL(min. The established system provides a highly efficient approach for high-throughput on-line clean-up of pyrethroid in various samples. (C) 2009 Elsevier B.V. All rights reserved.
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