Determination of deoxynivalenol in organic and conventional food and feed by sol-gel immunoaffinity chromatography and HPLC-UV detection

The paper describes the determination of deoxymvalenol (DON) in 55 wheat food anti feed samples. 26 from conventional anti 29 from organic production Immunoaffinity columns prepared by entrapping anti-DON antibodies by the sol-gel method were used for sample clean-up DON was quantified by high performance liquid chromatography (HPLC) and ultraviolet (UV) detection In general. the incidence of DON contamination was rather low In eight samples (14 5%) the DON concentration was above the LOQ (380 ng/g), in six samples (10 9%) DON was detected but could not be quantified (>LOD (200 ng/g). LOQ The data indicate both a higher incidence of DON contamination and higher DON concentrations in food and feed samples from conventional than in those front organic production (C) 2009 Elsevier B V All rights reserved