期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 877, 期 3, 页码 333-338出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2008.12.011
关键词
Capillary zone electrophoresis; Post-column derivatization; Laser-induced fluorescence; Aminoglycosides; Kanamycin A; Amikacin; Tobramycin
资金
- National Natural Science Foundation of China [20675075, 20275035]
An analytical method for kanamycin A, amikacin and tobramycin of aminoglycoside (AG) antibiotics in milk samples was proposed using capillary zone electrophoresis (CZE) with post-column derivatization and laser-induced fluorescence detection. A simple and convenient homemade coaxial-gap reactor was adopted in the post-column derivatization of the AGs with 1.0 mmol/L naphthalene-2,3-dicarboxaldehyde and 8.0 mmol/L 2-mercaptoethanol in 35 mmol/L sodium tetraborate buffer (pH 10.0) of 30% (v/v) methanol. 50 mmol/L sodium acetate (pH 5.0) containing 0.5 mmol/L cetyltrimethyl ammonium bromide was used as the separation buffer. The linear calibration concentrations and detection limits were from 2.1 X 10(-5) to 5.0 x 10(-2) g/L and in the range of 7 x 10(-6) to 2 x 10(-5) g/L, respectively. The recoveries of the AGs in milk samples were from 81.6 to 93.1% (n = 3). (c) 2008 Elsevier B.V. All rights reserved.
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