期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 877, 期 28, 页码 3378-3383出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2009.06.014
关键词
Thiols; Chemical modification; Fluorescence; Metallothionein; Zinc
资金
- National Institutes of Health
- John Sealy Memorial Endowment Fund
Fluorescence methods have been instrumental in demonstrating that the structure of human metallothionein in vivo depends on the availability of metal ions and the redox environment. Differential chemical modifications of its cysteine thiols with fluorescent probes allowed determination of three states: metallothionein (zinc-bound thiolate), thionein (free thiols), and thionin (disulfides). Interrogation of its zinc-binding properties with fluorescent chelating agents revealed that the affinities for the seven zinc ions vary over four orders of magnitude. Attachment of fluorescent labels generated metallothionein FRET (fluorescence resonance energy transfer) sensors for investigating its structure and function in living cells. (C) 2009 Elsevier B.V. All rights reserved.
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