期刊
NEUROREPORT
卷 26, 期 16, 页码 981-987出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/WNR.0000000000000455
关键词
age; dorsal root ganglion; HCN channels
资金
- National Natural Science foundation of China [30972848, 81271234]
- Special Funds of Basic research, operating expenses for universities of China [31500996, CZY14016, CZW15048, CZZ11009]
In rat's sensory neurons, hyperpolarization-activated inward currents (I-h) play an essential role in mediating action potentials and contributing to neuronal excitability. Classified by the size of neurons and ages, we studied the I-h and transcription levels of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels using electrophysiology and the single-cell RT-PCR. In voltage-clamp studies, I-h and half-maximal activation voltage (V-1/2) changed with age and size. An analysis of all HCN subtypes in dorsal root ganglion (DRG) neurons by single-cell RT-PCR was carried out. HCN1 and HCN3 in medium-small elderly neurons had a weak expression. HCN2 in newborns and HCN4 in elderly rats also had a weak expression. The aim of this study is to examine the age-related I-h and HCN channels subunits in different ages and sizes of DRG neurons. The results would be significant in understanding the physiological and pathophysiological function of different sizes of DRG neurons in different age periods. Copyright (C) 2015 Wolters Kluwer Health, Inc. All rights reserved.
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