期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 877, 期 3, 页码 351-354出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2008.12.044
关键词
Vitamin K-1; HPLC; Reference values; Haemostasis alteration; Bone mineralization
资金
- Italian Ministry of Research
We describe a procedure for quantification of vitamin K-1 in human plasma by HPLC. Samples, enriched with a vitamin K derivative as internal standard, were deproteinized, purified on polymeric RP-SPE cartridges and injected into HPLC equipped with a post-column on-line zinc metal reactor and a fluorometric detector. Median level in blood donors (n = 87) was 1.967 nmol/L(0.93-4.01, 5th-95th percentiles), with a significant correlation between plasma levels and age (r = 0.276, p = 0.00958) and a lower (not significant) value in women than in men. This method, easy-to-handle and with a high throughput, can be used to identify covert states of vitamin K intake deficiency in patients thus at risk of alterations in blood clotting or bone mineralization. (c) 2008 Elsevier B.V. All rights reserved.
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