期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 877, 期 29, 页码 3631-3637出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2009.09.004
关键词
Detonation nanodiamond; Sample pretreatment; Low-abundance peptides; Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
资金
- National Science and Technology Key Project of China [2007CB914100, 2009CB825607, 2008ZX10207]
- National Natural Science Foundation of China [20875016, 30672394, 30530040]
- Ministry of Education of China [20080246011]
- Shanghai Leading Academic Discipline [B109]
- Shanghai Projects [08DZ2293601]
Detonation nanodiamond (dND) was firstly employed as adsorbent for pretreatment of peptides in dilute/contaminated sample solution. Detonation nanodiamond showed high efficiency for isolating and enriching peptides in a wide pH range. Remarkably, good tolerance capability toward salts and detergents could be achieved by using dNDs. Due to the inherent specificities of dNDs, dND-bound peptides could be directly analyzed by MALDI-TOF MS, so as to avoid the elution step and reduce sample loss. This pretreatment method also exhibited a better performance for protein identification compared to solvent evaporation and Ziptip pretreatment approach. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.
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