期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 877, 期 26, 页码 2890-2894出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2009.01.002
关键词
Targeted lipidomics; N-acyl amino acids; Rat brain
资金
- National Institutes of Health/National Institute on Drug Abuse [DA16825, DA018224]
- Linda and Jack Gill Center for Biomolecular Science, Indiana University
- Indiana University, Bloomington
- Lilly Foundation Inc., Indianapolis, IN
Great effort has been devoted to characterize signaling lipids in central nervous system. This has led to a search for novel strategies to characterize hitherto unknown lipid compositions. Here we developed two methods, one for identification and one for quantification, for N-acyl amino acids, a novel lipid family. The identification method contains a series of purification steps followed by nano-LC/MS/MS and high-throughput screening of the datasets with a potent search algorithm based on fragment ion analysis. MS/MS spectra with good quality can be obtained with 150 fmol of targeted lipids on column with our nano-LC/MSIMS. More than one thousand mass spectra generated using the information dependent acquisition mode of Analyst QS software can be analyzed in 1 min using our home built software. The quantification method utilized the multiple reaction monitoring mode in Analyst software to measure the endogenous levels of N-acyl amino acids in rat brain. Using these two methods we were able to identify and quantify 11 previously reported N-acyl amino acids with endogenous levels ranging from 0.26 to 333 pmol g(-1) wet rat brain. Published by Elsevier B.V.
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