期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 877, 期 10, 页码 975-982出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2009.02.035
关键词
Celiac disease; Gluten protein extraction; Gliadins; Glutenins; Immunoblotting; Monoclonal antibodies; Wheat; R5
资金
- Celiac Disease Consortium
- Dutch Government [BSIK03009]
- Dutch Ministry of Agriculture, Nature, and Food Safety [KB-05-001-019-PRI]
The detection, analysis, and quantification of individual celiac disease (CD) immune responsive gluten proteins in wheat and related cereals (barley, rye) require an adequate and reliable extraction protocol. Because different types of gluten proteins behave differently in terms of solubility, currently different extraction protocols exist. The performance of various documented gluten extraction protocols is evaluated for specificity and completeness by gel electrophoresis (SDS-PAGE), immunoblotting and RIDASCREEN (R) Gliadin competitive ELISA. Based on these results, an optimized, two-step extraction protocol has been developed. (C) 2009 Elsevier B.V. All rights reserved.
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