Monodispersed, molecularly imprinted polymers as affinity-based chromatography media

This review article deals with preparation methods for spherical and monodispersed molecularly imprinted polymers (MIPs) in micrometer sizes. Those methods include suspension polymerization in water, liquid perfluorocarbon and mineral oil, seed polymerization and dispersion/precipitation polymerization. The other methods are the use of beaded materials such as a spherical silica or organic polymer for grafting MIP phases onto the surfaces of porous materials or filling the pores of silica with MIPs followed by dissolution of the silica. Furthermore, applications of MIP microspheres as affinity-based chromatography media, HPLC stationary phases and solid-phase extraction media, will be discussed for pharmaceutical, biomedical and environmental analysis. (C) 2007 Elsevier B.V. All rights reserved.