期刊
出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2008.01.015
关键词
jasmonic acid methyl ester; salicylic acid methyl ester; indole 3-acetic acid methyl ester; carboxyl methyl esterase; gas chromatography-mass spectroscopy
We developed a quantitative method for the determination of methyl esterase activity, analyzing substrate specificity against three major signal molecules, jasmonic acid methyl ester (MeJA), salicylic acid methyl ester (MeSA), and indole-3-acetic acid methyl ester (MeIAA). We used a silylation reagent for chemical derivatization and used gas chromatography (GC)-mass spectroscopy in analyses, for high precision. To test this method, an Arabidopsis esterase gene, AtME8, was expressed in Escherichia coli, and then the kinetic parameters of the recombinant enzyme were determined for three substrates. Finally, this method was also applied to the direct quantification of phytohormones in petals from lilies and roses. (C) 2008 Elsevier B.V. All fights reserved.
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