期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 874, 期 1-2, 页码 115-118出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2008.09.003
关键词
Amphetamines; GC-MS; Microwave-assisted derivatization; Solvent; Urine
资金
- National Science Council of the Republic of China, Taiwan [NSC 94-2113-M-005-001]
An approach using microwave-assisted derivatization (MAD) following solid-phase extraction (SPE) combined with gas chromatography-mass spectrometry (GC-MS) was developed to determine amphetamines in urine samples. The parameters affecting the derivatization efficiency - including microwave power and irradiation time - were investigated. Besides, solvent is thought critically important to MAD. Derivatization performance was studied using various solvents and compared with the performance obtained without solvent. Derivatization efficiency was clearly found to be enhanced by the presence of solvent. The highest derivatization efficiencies were obtained in ethyl acetate (EA) under microwave power of 250 W for I min. Calibration curves for all amphetamines were linear over a range from I to 1000 ng/mL, with correlation coefficients above 0.9992. The intra-day and inter-day precision were less than 15%. The applicability of the method was tested by analyzing amphetamine-abusing subjects urine samples. Accordingly, the solvent-enhanced MAD-CC-MS method appears to be adequate for determining amphetamines in urine. (C) 2008 Elsevier B.V. All rights reserved.
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