期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 874, 期 1-2, 页码 21-26出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2008.08.025
关键词
Affinity chromatography; Ligand; Peptide; Plasmid DNA purification; Single step purification
资金
- VESKI, the Victorian Endowment for Science, Knowledge and Information
Single step affinity chromatography was employed for the purification of plasmid DNA (pDNA), thus eliminating several steps compared with current commercial purification methods for pDNA. Significant reduction in pDNA production time and cost was obtained. This chromatographic operation employed a peptide-monolith construct to isolate pDNA from Escherichia coli (E coli) impurities present in a clarified lysate feedstock. Mild conditions were applied to avoid any degradation of pDNA. The effect of some important parameters on pDNA yield was also evaluated with the aim of optimising the affinity purification of pDNA. The results demonstrate that 81% of pDNA was recovered and contaminating gDNA, RNA and protein were removed below detectable levels. (C) 2008 Elsevier B.V. All rights reserved.
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