期刊
出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2008.06.001
关键词
thin-layer chromatography; matrix-assisted laser desorption/ionization; glycosphingolipid
资金
- NINDS NIH HHS [R01 NS009626, R01 NS009626-36S1, R01 NS009626-36, R01NS009626, R01 NS009626-36S2] Funding Source: Medline
Glycosphingolipids are ubiquitous constituents of cells. Yet there is still room for improvement in the techniques for analyzing glycosphingolipids. He re we report our highly sensitive and convenient analytical technology with imaging mass spectrometry for detailed structural analysis of glycosphingolipids. We were able to determine detailed ceramide structures; i.e., both the sphingosine base and fatty acid, by MS/MS/MS analysis on a PVDF membrane with 10 pmol of GM1, with which only faint bands were visible by primuline staining. The limit of detection was approximately 1 pmol of GM1, which is lower than the value in the conventional reports (10 pmol). (C) 2008 Elsevier B.V. All rights reserved.
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