期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 875, 期 1, 页码 142-147出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2008.05.018
关键词
HPLC; UV-detection; Stereospecific; Enantiomer; Flavonoid
资金
- NIGMS NIH HHS [T32 GM008336-16, T32 GM008336-18, T32 GM008336-17] Funding Source: Medline
A stereospecific method of analysis of racemic isosakuranetin (5,7-dihydroxy-4'-methoxyflavanone) in biological fluids is necessary to study pharmacokinetics. A simple high-performance liquid chromatographic method was developed for the determination of isosakuranetin enantiomers. Separation was achieved on a Chiralpak (R) AD (TM)-RH column with ultraviolet (UV)-detection at 286 nm. The standard curves in urine were linear ranging from 0.5 to 100.0 mu g/ml for each enantiomer. The mean extraction efficiency was > 88.0%. Precision of the assay was < 15% (CV) and was within 12% at the limit of quantitation (0.5 mu g/ml). Bias of the assay was < 15% and was within 6% at the limit of quantitation. The assay was applied successfully to stereospecific disposition of isosakuranetin enantiomers in rat urine. (c) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据