期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 872, 期 1-2, 页码 181-185出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2008.07.030
关键词
counter-current chromatography; Aconitum coreanum; preparative separation and purification; isomeric diterpenoid alkaloids
Preparative high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was used to isolate and separate bioactive constituents from the roots of Aconitum coreanum. Two new diterpenoid alkaloid isomers were successfully separated for the first time by HSCCC with an optimized two-phase solvent system composed of ethyl acetate-n-butanol-methanol-2% acetic acid (3.5:1.5:2:4.5, v/v/v/v), 25.4 mg of GFT (1) and 18.3 mg of GFU (2) were isolated form 1 g crude extract in one step HSCCC experiment. The purities of the two new compounds were all over 95% as analyzed by HPLC and their structures were identified by ESI-MS, H-1 NMR, C-13 NMR, and 2D NMR analysis. (c) 2008 Elsevier B.V. All rights reserved.
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