期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1358, 期 -, 页码 208-216出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2014.07.003
关键词
Capillary electrochromatography; Vancomycin; Monolithic column; Sol-gel process; Chiral separation
资金
- National Science Council of ROC [NSC 96-2113-M-194-012-MY3]
- National Chung Cheng University
A vancomycin-bonded silica monolithic column for capillary electrochromatography (CEC) was prepared by a single-step in situ sol-gel approach. This sol-gel process incorporates a synthetic sol-gel precursor which contains a macrocyclic antibiotic, vancomycin, to form a porous silica network inside a fused-silica capillary. To avoid degradation of vancomycin during the column fabrication, a mild step was adopted into the sal-gel process. The performance of the vancomycin chiral stationary phase was investigated by CEC in both the reversed-phase mode and the normal-phase mode. The vancomycin chiral stationary phase was optimized with respect to vancomycin loading in the reversed-phase mode for chiral separation of thalidomide enantiomers. The best efficiency and resolution values of 94 600 plates/m and 5.79, respectively, were achieved. The optimized column was further applied to chiral separation of alprenolol enantiomers. A plate height of less than 7 pm for the first eluted enantiomer of alprenolol was obtained in an aqueous mobile phase at a flow rate of 0.74 mm/s. Using enantiomers of seven beta-blockers and some other basic enantiomers as test analytes, separation efficiencies of up to 148 100 plates/m in the reversed-phase mode and up to 138 100 plates/m in the normal-phase mode were achieved. (C) 2014 Elsevier B.V. All rights reserved.
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