期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1326, 期 -, 页码 1-6出版社
ELSEVIER
DOI: 10.1016/j.chroma.2013.12.037
关键词
Micro extraction; Spermidine; Spermine; Urine; Blood
资金
- National Science Council [NSC 102-2113-M-037-010]
- NSYSU-KMU JOINT RESEARCH PROJECT [NSYSUKMU102-P010]
- Center of Excellence for Environmental Medicine (Kaohsiung Medical University)
Spermidine and its derivative, spermine, are basic compounds with unique roles in physiological function. This study used matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to monitor these analytes in human urine and blood. The MALDI-TOF MS is also suitable when a high-throughput analytical method is required. Unlike liquid chromatography (LC)-MS, MALDI-TOF MS does not require a mobile phase in sample separation and generates very little organic waste. Micro liquid-liquid extraction was also performed to minimize the use of organic solvents in this study. After alkalization step, biosamples were prepared by adding a small volume (20 mu L) of organic solvent to concentrate, and purify the spermidine and spermine contained in the urine and blood samples. A suitable extraction protocol was developed after optimizing various conditions associated with extraction efficiency. The proposed method was then successfully used to monitor these compounds in human urine (mean value < 1 mu g/mL) and blood (mean value > 1 mu g/mL). (C) 2013 Elsevier B.V. All rights reserved.
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