期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1364, 期 -, 页码 214-222出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2014.08.088
关键词
Liquid chromatography-mass spectrometry; Atmospheric pressure photoionization; Vitamin D; Oxysterols; Cell line samples; Mouse brain samples
资金
- FinPharma Doctoral Program (FPDP)
- Waldemar von Frenckell Foundation
- Magnus Ehrnrooth Foundation
- Academy of Finland [251575]
- Netherlands Metabolomics Centre (NMC), the Netherlands Genomics Initiative/Netherlands Organization for Scientific Research
- Dutch Technology Foundation (STW)
- Academy of Finland (AKA) [251575, 251575] Funding Source: Academy of Finland (AKA)
We have developed an ultra-high-performance liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometric (UHPLC-APPI-MS/MS) method for the simultaneous quantitative analyses of several oxysterols and vitamin D metabolites in mouse brain and cell line samples. An UHPLC-APPI-high resolution mass spectrometric (UHPLC-APPI-HRMS) method that uses a quadrupole-time of flight mass spectrometer was also developed for confirmatory analysis and for the identification of non-targeted oxysterols. Both methods showed good quantitative performance. Furthermore, APPI provides high ionization efficiency for determining oxysterols and vitamin D related compounds without the time consuming derivatization step needed in the conventionally used electrospray ionization method to achieve acceptable sensitivity. Several oxysterols were quantified in mouse brain and cell line samples. Additionally, 25-hydroxyvitamin D-3 was detected in mouse brain samples for the first time. (C) 2014 Elsevier B.V. All rights reserved.
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