期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1340, 期 -, 页码 90-98出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2014.03.020
关键词
Practical peak capacity; Two-dimensional liquid chromatography; Biomass; Bio-oil; RPLC x RPLC
Comprehensive on-line two-dimensional liquid chromatography (on-line LC x LC) was used for the characterization of bio-oils obtained by fast pyrolysis of lignocellulosic biomass. The resulting bio-oil contains a large number of oxygenated chemical families and must therefore be upgraded before being used as drop-in transportation biofuels. The good knowledge of its complex composition is essential for optimizing the mandatory bio-oil upgrading process to biofuels, thereby requiring powerful separation techniques designed to be hyphenated to mass spectrometry detection (LC x LC-MS). In this study, reversed phase conditions were optimized in both dimensions for the RPLC x RPLC separation of the aqueous fraction of bio-oils. The first step of method development consisted in searching for a suitable set of RP-conditions via the screening of a large number of RP-systems (made up of different stationary phases and/or mobile phases and/or temperature). The practical peak capacity and the degree of orthogonality were calculated for a sample of 38 representative compounds, both descriptors having been considered as selection criterion. Two different couplings were chosen and evaluated for the RPLC x RPLC separation of the 38 representative compounds. The best of both, in terms of real practical peak capacity, was further successfully applied to the separation of the aqueous phase of a partially dehydroxygenated bio-oil. (C) 2014 Elsevier B.V. All rights reserved.
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