期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1363, 期 -, 页码 119-127出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2014.08.040
关键词
Chiral separation; Cyclodextrin; Chiral stationary phase; Reversed phase-HPLC
资金
- National Natural Science Foundation of China [21305066]
- Program for New Century Excellent Talents in University [NCET-12-0633]
- Ministry of Education of China [20103219120008]
- Jiangsu Province Natural Science Fund for Distinguished Young Scholars [BK20130032]
- Fundamental Research Funds for the Central Universities [30920130111006]
In this study, perphenylcarbamated cyclodextrin clicked chiral stationary phase (CSP) was developed with high column efficiency. The characteristics of the column were evaluated in terms of linearity, limit of detection and limit of quantification. The enantioselectivity of the as-prepared clicked CSP was explored with 26 recemates including aryl alcohols, flavanoids and adrenergic drugs in reversed phase high-performance liquid chromatography. The effect of separation parameters including flow rate, column temperature, organic modifier and buffer on the enantioselectivity of the clicked CSP was investigated in detail. The correlation study of the analytes structure and their chiral resolution revealed the great influence of analytes' structure on the enantioseparations with cyclodextrin CSP. Methanol with 1% of triethylammonium acetate buffer (pH 4) was proved to be the best choice for the chiral separation of basic enantiomers. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据