期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1313, 期 -, 页码 284-290出版社
ELSEVIER
DOI: 10.1016/j.chroma.2013.06.052
关键词
Molecularly imprinted polymer; Solid-phase extraction; Capillary HPLC-ESI ion trap MS/MS; (E)-Resveratrol; Pinot noir red wine
资金
- Centre for Green Chemistry of Monash University
- Ministry of Science, Technology and Innovation of Malaysia
Red wine has long been credited as a good source of health-beneficial antioxidants, including the bioactive polyphenols catechin, quercetin, and (E)-resveratrol. In this paper, we report the application of reusable molecularly imprinted polymers (MIPs) for the selective and robust solid-phase extraction (SPE) and rapid analysis of (E)-resveratrol (LOD = 8.87 x 10(-3) mg/L, LOQ= 2.94 x 10(-2) mg/L), along with a range of other polyphenols from an Australian Pinot noir red wine. Optimization of the molecularly imprinted solid-phase extraction (MISPE) protocol resulted in the significant enrichment of (E)-resveratrol and several structurally related polyphenols. These secondary metabolites were subsequently identified by RP-HPLC and mu LC-ESI ion trap MS/MS methods. The developed MISPE protocol employed low volumes of environmentally benign solvents selected according to the Green Chemistry principles, and resulted in the recovery of 99% of the total (E)-resveratrol present. These results further demonstrate the potential of generic protocols for the analysis of target compound with health beneficial properties within the food and nutraceutical industries using tailor-made MIPs. (C) 2013 Elsevier B.V. All rights reserved.
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