期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1287, 期 -, 页码 97-112出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2013.02.028
关键词
Flow field-flow fractionation; Retention level; Ultrafiltration membrane; Relaxation/focusing time; Membrane fouling; Sample loss
资金
- Swedish Research Council
This overview regards some critical issues in performing flow field-flow fractionation (flow FFF, FlFFF, AF4, HF-FlFFF, HF5). It includes the channel thickness, void time, channel-flow parabolic profile, channel-flow velocity gradient, uniformity of the cross-flow, sample injection time, relaxation/focusing time, width of sample starting zone, retention level, theoretical and experimental zone broadening, hydrodynamic threshold immobilisation/re-mobilisation, sample loss and adsorption, membrane fouling, sample mass overloading, problems with symmetrical channels, non-spherical sample particles, and method development. Good method development practice (GMDP) and good fractogram practice (GFP) is suggested. (C) 2013 Elsevier B.V. All rights reserved.
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