期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1272, 期 -, 页码 20-25出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2012.11.059
关键词
Protein chromatography; Composite cryogel; Particle embedding; Adsorption capacity; Anion-exchange
资金
- Natural Science Foundation of China [20976126, 21236005]
Novel composite cryogel monoliths were fabricated by incorporating polymeric resin particles and grafting anion-exchange groups on the pore wall surfaces. The embedded resin particles in different size distributions were prepared by grinding poly(glycidyl methacrylate-ethylene glycol dimethacrylate) monoliths. Observations by scanning electron microscopy clearly indicated that the composite cryogel had interconnected large pores (10-100 mu m in width), similar to normal (pure) cryogel. However, the composite material had very rough pore walls, which provided larger surface area for protein adsorption. As a result, the dynamic binding capacity of bovine serum albumin (BSA) on the composite cryogel bed reached 6 mg/mL bed (flow velocity, 5 cm/min), which was 2.8 times higher than a cryogel bed without embedding the ground resin particles. The capacity value was also much higher than the BSA capacities of cryogel beds reported in literature (1-4 mg/mL). Though the capacity decreased by about 1 mg/mL with the increase of flow rate from 0.5 to 5 cm/min, it then kept almost unchanged till a flow rate up to 15 cm/min. The height equivalent to a theoretical plate of the composite bed was in the range of 1.1-1.4 mm, and kept nearly constant in a flow rate range of 5-20 cm/min. The results indicated that the composite cryogel bed offered a large improvement in protein adsorption capacity and was suitable for high-speed protein chromatography. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据